Catalog No: OKEH03160
Size:96T
Price: $525.00
SKU
OKEH03160
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SST ELISA Kit (Rat) (OKEH03160) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Sst antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Sst and incubated. The Sst found in the sample or standards competes with the biotinylated Sst for limited binding sites on the immobilized anti-rat Sst antibody. Excess unbound biotinylated Sst and sample or standard Sst is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Sst captured in the well and inversely proportional to the amount of Sst which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into serum: 100%
ELISA Kit ReproducibilityIntra-Assay CV: ≤5.4% (n=20)
Inter-Assay CV: ≤6.7% (n=24)
ELISA Kit Component
ComponentAmount
Anti-Sst Microplate96 Wells (12 x 8 Well strips)
Sst Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Sst-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Rat Somatostatin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolSst
Gene Full Namesomatostatin
Alias SymbolsSmst;somatostatin;somatotropin release-inhibiting factor;SRIF;SS-14;SS-28.
NCBI Gene Id24797
Protein NameP60042
Description of TargetSomatostatin
Uniprot IDhttps://www.uniprot.org/uniprot/P60042
Protein Accession #NP_036791.1
Nucleotide Accession #NM_012659.2
Protein Size (# AA)116
Molecular Weight13 kDa
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