Catalog No: OKEH04355
Size:96T
Price: $550.00
SKU
OKEH04355
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04355
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Sst ELISA Kit (Mouse) (OKEH04355) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sst has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Sst is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sst captured in the well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.1% (n = 20)
Mean Inter-assay CV%: <= 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Sst Microplate96 Wells (12 x 8 Well Strips)
Sst Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Sst Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity4.22 pg/mL
SpecificityNatural and recombinant Mouse Somatostatin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSst
Gene Full Namesomatostatin
Alias Symbolspreprosomatostatin;S;Sm;Smst;SOM;somatostatin;SRIF;SS.
NCBI Gene Id20604
Protein NameP60041
Description of TargetSomatostatin
Uniprot IDhttps://www.uniprot.org/uniprot/P60041
Protein Accession #NP_033241.1
Nucleotide Accession #NM_009215.1
Protein Size (# AA)116
Molecular Weight13 KDa
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