Catalog No: OKEH02913
Price: $600.00
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SST ELISA Kit (Human) : 96 Wells (OKEH02913)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02913
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SST ELISA Kit (Human) (OKEH02913) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SST antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SST and incubated. The SST found in the sample or standards competes with the biotinylated SST for limited binding sites on the immobilized anti-human SST antibody. Excess unbound biotinylated SST and sample or standard SST is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SST captured in the well and inversely proportional to the amount of SST which was contained in the sample or standard.
ELISA Kit Range6.25-400pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.7% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
Anti-SST Microplate96 Wells (12 x 8 Well strips)
SST Lyophilized Standard2
Sample Diluent1 x 20 mL
100X SST-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity1.56 pg/mL
SpecificityNatural and recombinant Human Somatostatin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolSST
Gene Full Namesomatostatin
Alias Symbolsgrowth hormone release-inhibiting factor;prepro-somatostatin;SMST;somatostatin;somatostatin-14;somatostatin-28;SST1.
NCBI Gene Id6750
Protein NameP61278
Description of TargetSomatostatin
Uniprot ID
Protein Accession #NP_001039.1
Nucleotide Accession #NM_001048.3
Protein Size (# AA)116
Molecular Weight13 kDa
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