Catalog No: OPCA02060
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OPCA02060
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Product Info
Predicted Species ReactivitySulfolobus solfataricus
Product FormatLyophilized 20mM Tris-HCl, 0.5M NaCl, 6% Trehalose
HostSulfolobus solfataricus
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
PurificationAffinity purified using IMAC
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequenceFull Length of Mature Protein: ATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK
SourceE.coli
TagN-terminal 6xHis-SUMO-tagged
ReferenceThe complete genome of the crenarchaeon Sulfolobus solfataricus P2.She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001)
Gene SymbolSSO7D
Alias Symbols7 kDa DNA-binding protein d;Sso7d.
NCBI Gene Id25402805
Protein NameDNA-binding protein 7d
Description of TargetCan constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).
Uniprot IDP39476
Protein Accession #WP_009990119.1
Nucleotide Accession #NC_002754.1
Protein Size (# AA)2-64
Molecular Weight23.1 kDa
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