SSC5D ELISA Kit (Human) (OKWB00228)

Catalog No: OKWB00228

Size:96T

Human SSC5D ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	93-102%	86-105%	85-103%	87-105%
  EDTA Plasma (n=5)	85-96%	85-100%	84-101%	86-96%
  Heparin Plasma (n=5)	86-99%	81-99%	86-100%	81-98%

• ELISA Kit Principle: Aviva Systems Biology SSC5D ELISA Kit (Human) (OKWB00228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SSC5D has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SSC5D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SSC5D captured in the well.
• ELISA Kit Range: 0.313-20ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	85-104	95
  EDTA Plasma (n=5)	88-105	97
  Heparin Plasma (n=5)	87-105	97

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • SSC5D Microplate: 96 Wells (12 x 8 Well strips)
  • SSC5D Lyophilized Standard: 2 x 20 ng
  • 100X SSC5D Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SSC5D
• Gene Full Name: scavenger receptor cysteine rich family member with 5 domains
• Alias Symbols: S5D-SRCRB;scavenger receptor cysteine rich domain containing (5 domains);scavenger receptor cysteine rich family, 5 domains;scavenger receptor cysteine-rich glycoprotein;soluble scavenger protein with 5 SRCR domains;soluble scavenger receptor cysteine-rich domain-containing protein SSC5D;soluble scavenger with 5 domains.
• NCBI Gene Id: 284297
• Protein Name: Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D
• Description of Target: Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces (By similarity).
• Uniprot ID: A1L4H1
• Protein Accession #: NP_001138422
• Nucleotide Accession #: NM_001144950