SRSF1 Recombinant Protein (OPCD00547)

Catalog No: OPCD00547

SRSF1 Recombinant Protein (OPCD00547) using SDS-PAGE

SRSF1 Recombinant Protein (OPCD00547) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Cys16-Arg224
  Subcellular Location: Nucleus, Cytoplasm
  Isoelectric Point: 9.9
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 300 ug/mL (prior to lyoph)
• Purity: > 97%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: SRSF1
• Gene Full Name: serine and arginine rich splicing factor 1
• Alias Symbols: alternative-splicing factor 1;ASF;pre-mRNA-splicing factor SF2, P33 subunit;serine/arginine-rich splicing factor 1;SF2;SF2p33;SFRS1;splicing factor 2;Splicing factor, arginine/serine-rich 1;splicing factor, arginine/serine-rich, 30-KD, A;SR splicing factor 1;SRp30a.
• NCBI Gene Id: 6426
• Protein Name: Serine/arginine-rich splicing factor 1
• Description of Target: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
• Uniprot ID: Q07955
• Protein Accession #: NP_001071634.1
• Nucleotide Accession #: NM_001078166.1
• Protein Size (# AA): Cys16~Arg224
• Molecular Weight: 27kDa