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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for SRSF1 Antibody (OACD00859) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Range: Cys16 - Arg224 |
| Purification | Affinity Purified; Protein A Purified |
| Concentration | 0.5 mg/ml |
| Preservative | 0.02% NaN3 |
| Specificity | The antibody is a rabbit polyclonal antibody raised against SRSF1. It has been selected for its ability to recognize SRSF1 in immunohistochemical staining and western blotting. |
| Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
| Gene Symbol | SRSF1 |
|---|---|
| Gene Full Name | serine and arginine rich splicing factor 1 |
| Alias Symbols | alternative-splicing factor 1;ASF;pre-mRNA-splicing factor SF2, P33 subunit;serine/arginine-rich splicing factor 1;SF2;SF2p33;SFRS1;splicing factor 2;Splicing factor, arginine/serine-rich 1;splicing factor, arginine/serine-rich, 30-KD, A;SR splicing factor 1;SRp30a. |
| NCBI Gene Id | 6426 |
| Protein Name | Serine/arginine-rich splicing factor 1 |
| Description of Target | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
| Uniprot ID | Q07955 |
| Protein Accession # | NP_001071634.1 |
| Nucleotide Accession # | NM_001078166 |
| Molecular Weight | 22 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SRSF1 Antibody (OACD00859)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "SRSF1 Antibody (OACD00859)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "SRSF1 Antibody (OACD00859)" provided in?
This item is provided in "Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SRSF1 Antibody (OACD00859)"?
This target may also be called "alternative-splicing factor 1;ASF;pre-mRNA-splicing factor SF2, P33 subunit;serine/arginine-rich splicing factor 1;SF2;SF2p33;SFRS1;splicing factor 2;Splicing factor, arginine/serine-rich 1;splicing factor, arginine/serine-rich, 30-KD, A;SR splicing factor 1;SRp30a." in publications.
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What is the shipping cost for "SRSF1 Antibody (OACD00859)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SRSF1 Antibody (OACD00859)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SRSF1 Antibody (OACD00859)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "22 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SRSF1 Antibody (OACD00859)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SRSF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SRSF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SRSF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SRSF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SRSF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SRSF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
