SRSF1 Antibody (OACA07055)

Catalog No: OACA07055

Immunohistochemistry of paraffin-embedded human breast cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human breast cancer at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Serine/arginine-rich splicing factor 1 protein (1-248AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006). [2]"A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1."Ngo J.C., Giang K., Chakrabarti S., Ma C.T., Huynh N., Hagopian J.C., Dorrestein P.C., Fu X.D., Adams J.A., Ghosh G.Mol. Cell 29:563-576(2008). [3]"Solution structure of RRM domain in splicing factor SF2."RIKEN structural genomics initiative (RSGI)Submitted (NOV-2005).

Target Info

• Gene Symbol: SRSF1
• Gene Full Name: serine and arginine rich splicing factor 1
• Alias Symbols: alternative-splicing factor 1;ASF;pre-mRNA-splicing factor SF2, P33 subunit;serine/arginine-rich splicing factor 1;SF2;SF2p33;SFRS1;splicing factor 2;Splicing factor, arginine/serine-rich 1;splicing factor, arginine/serine-rich, 30-KD, A;SR splicing factor 1;SRp30a.
• NCBI Gene Id: 6426
• Protein Name: Serine/arginine-rich splicing factor 1
• Description of Target: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
• Uniprot ID: Q07955
• Protein Accession #: NP_001071634
• Nucleotide Accession #: NM_001078166