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Datasheets/Manuals | Printable datasheet for SRPK1 Rabbit pAb (OAAN01583) |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH 7.3 |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Western blot |
Additional Information | Cellular Location: Cytoplasm, Microsome, Microsome, Nucleus, Nucleus matrix Positive Samples: 293T, HepG2, MCF7, HeLa, THP-1, Rat testis, Mouse spleen |
Reconstitution and Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
Immunogen | SRPK1 |
Purification | Affinity purified against immunogen |
Intended Use | For research purposes only. Not for therapeutic or diagnostic purposes in humans or animals. |
Application Info | WB: 1:500~2000 IHC: 1:50~200 IF: 1:10~100 |
Protein Sequence | EEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLV |
Gene Symbol | SRPK1 |
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Gene Full Name | SRSF protein kinase 1 |
Alias Symbols | Serine/arginine-rich protein-specific kinase 1;serine/arginine-rich splicing factor kinase 1;serine/threonine-protein kinase SRPK1;SFRS protein kinase 1;SFRSK1;SR-protein-specific kinase 1;SRSF protein kinase 1. |
NCBI Gene Id | 6732 |
Protein Name | SRSF protein kinase 1 |
Description of Target | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation. |
Uniprot ID | Q96SB4 |
Protein Accession # | NP_003128.3 |
Nucleotide Accession # | NM_003137.4 |
Molecular Weight | 74kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SRPK1 Rabbit pAb (OAAN01583)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "SRPK1 Rabbit pAb (OAAN01583)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "SRPK1 Rabbit pAb (OAAN01583)" provided in?
This item is provided in "Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH 7.3".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SRPK1 Rabbit pAb (OAAN01583)"?
This target may also be called "Serine/arginine-rich protein-specific kinase 1;serine/arginine-rich splicing factor kinase 1;serine/threonine-protein kinase SRPK1;SFRS protein kinase 1;SFRSK1;SR-protein-specific kinase 1;SRSF protein kinase 1." in publications.
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What is the shipping cost for "SRPK1 Rabbit pAb (OAAN01583)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SRPK1 Rabbit pAb (OAAN01583)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SRPK1 Rabbit pAb (OAAN01583)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "74kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SRPK1 Rabbit pAb (OAAN01583)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SRPK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SRPK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SRPK1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SRPK1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SRPK1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SRPK1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.