SRGN ELISA Kit (Mouse) (OKCD08282)

Catalog No: OKCD08282

Size:96WELLS

SRGN ELISA Kit (Mouse) (OKCD08282) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serglycin (SRGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serglycin (SRGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serglycin (SRGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serglycin (SRGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serglycin (SRGN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serglycin (SRGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SRGN Microplate: 96 Wells (12 x 8 Well strips)
  • SRGN Lyophilized Standard: 2
  • 100X SRGN HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 20.14pg/mL

Target Info

• Gene Symbol: Srgn
• Gene Full Name: serglycin
• Alias Symbols: gp600;mastocytoma proteoglycan core protein;Pr;Prg;Prg1;proteoglycan 1, secretory granule;proteoglycan, secretory granule;secretory granule proteoglycan core protein;serglycin;Sg;Sgc.
• NCBI Gene Id: 19073
• Protein Name: Serglycin
• Description of Target: Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
• Uniprot ID: P13609
• Protein Accession #: NP_035287.1
• Nucleotide Accession #: NM_011157.2