Catalog No: OKEH08647
Size:96T
Price: $875.00
SKU
OKEH08647
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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SRF ELISA Kit (Chicken) (OKEH08647)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology SRF ELISA Kit (Chicken) (OKEH08647) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SRF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SRF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SRF captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.3% (n = 20)
Mean Inter-assay CV%: <=12.3% (n = 20)
ELISA Kit Component
ComponentAmount
SRF Microplate96 Wells (12 x 8 Well strips)
SRF Lyophilized Standard2
100X Biotinylated SRF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as c-fos). Required for cardiac differentiation and maturation.
::Subcellular Location: Nucleus
::Unigene: Gga.2013
String: 9031.ENSGALP00000021614
::Pfam: PF00319
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Chicken Serum response factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSRF
Alias SymbolsSRF
Protein NameQ90718
Description of TargetSerum response factor
Uniprot IDhttps://www.uniprot.org/uniprot/Q90718
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