SREBF1 Antibody - middle region (AVARP09051_P050)

Catalog No: AVARP09051_P050

WB Suggested Anti-SREBF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T

Human Liver

Rabbit Anti-SREBF1 Antibody
Catalog Number: AVARP09051_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Nucleus in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Goat, Horse
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SREBF1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 88%; Goat: 88%; Horse: 80%; Human: 82%; Mouse: 93%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: DAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSR
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-SREBF1 (AVARP09051_P050) antibody is Catalog # AAP30613 (Previous Catalog # AAPP01266)
• Reference: Motoyama,K., (2006) Biochem. Biophys. Res. Commun. 342 (1), 354-360

Target Info

• Gene Symbol: SREBF1
• Gene Full Name: Sterol regulatory element binding transcription factor 1
• Alias Symbols: HMD, IFAP2, SREBP1, bHLHd1
• NCBI Gene Id: 6720
• Protein Name: Sterol regulatory element-binding protein 1
• Description of Target: SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P36956
• Protein Accession #: NP_001005291
• Nucleotide Accession #: NM_001005291
• Protein Size (# AA): 1177
• Molecular Weight: 125kDa
• Protein Interactions: UBC; ERLIN2; FBXW7; SMARCD3; HSP90AA1; SUMO1; ATXN1L; INSIG2; SCAP; ATXN1; LMNA; INSIG1; EGF; SREBF2; SREBF1; ZBTB7A; ZBTB7C; SUMO2; MED6; MED13; MED12; MED24; MED26; MED17; MED23; MED14; USF1; MED1; GSK3B; CREBBP; CDK8; MED25; MED15; MED7; MED21; SIRT1;