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SRD5A1 ELISA Kit (Rat) (OKCD02894)

Catalog#: OKCD02894
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 3 Hours
Kit Principle Aviva Systems Biology Srd5a1 ELISA Kit (Rat) (OKCD02894) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Srd5a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Srd5a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Srd5a1 captured in well.
Kit Range 0.156-10 ng/mL
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level SRD5A1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SRD5A1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Component
Anti-SRD5A1 Microplate96 Wells (12 x 8 Well strips)
SRD5A1 Lyophilized Standard2
100X Biotinylated SRD5A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Tissue homogenates, cell lysates and other biological fluids
Sensitivity 0.058 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 1 (SRD5a1). No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 1 (SRD5a1) and analogues was observed
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol SRD5A1
Official Gene Full Name steroid 5 alpha-reductase 1
Alias Symbols 3-oxo-5-alpha-steroid 4-dehydrogenase 1, MGC156498, S5AR 1, SR type 1, Steroid 5-alpha-reductase 1
NCBI Gene Id 24950
Protein Name 3-oxo-5-alpha-steroid 4-dehydrogenase 1
Description of Target Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Swissprot Id P24008
Protein Accession # NP_058766.2
Nucleotide Accession # NM_017070.3
Protein Size (# AA) 259
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express SRD5A1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express SRD5A1.
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