Catalog No: OCOA19065
Size:20UG
Price: $169.00
SKU
OCOA19065
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SQSTM1 Protein Lysate (OCOA19065)

Datasheets/ManualsPrintable datasheet for SQSTM1 Protein Lysate (OCOA19065)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSQSTM1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSQSTM1
Gene Full Namesequestosome 1
Alias SymbolsA170;autophagy receptor p62;DMRV;EBI3-associated protein of 60 kDa;EBI3-associated protein p60;EBIAP;FTDALS3;NADGP;OSIL;oxidative stress induced like;p60;p62;p62B;PDB3;phosphotyrosine independent ligand for the Lck SH2 domain p62;phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;sequestosome-1;ubiquitin-binding protein p62;ZIP3.
NCBI Gene Id8878
Protein NameNM_001142298, NP_001135770, Q13501
Description of TargetHomo sapiens sequestosome 1 (SQSTM1), transcript variant 2, mRNA, Sequestosome-1, sequestosome-1 isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q13501
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001135770
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001142298
Molecular Weight47.69 kDa
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