SQSTM1/P62 Antibody - N-terminal region (OABB01927)

Catalog No: OABB01927

Size:100UG

Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (OABB01927). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: COS7 whole cell lysates
Lane 3: A549 whole cell lysates,
Lane 4: U87 whole cell lysates
Lane 5: Hela whole cell lysates.
Lane 6: Caco-2 whole cell lysates.
Lane 7: Hepg2 whole cell lysates.
Lane 8: THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 antigen affinity purified polyclonal antibody (Catalog # OABB01927) at 0.5 ug/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62KD. The expected band size for SQSTM1 is at 62KD.

Anti-SQSTM1 Picoband antibody, OABB01927, IHC(P)
IHC(P): Human Lung Cancer Tissue

Anti-SQSTM1 Picoband antibody, OABB01927, IHC(F)
IHC(F): Mouse Spleen Tissue

Anti-SQSTM1 Picoband antibody, OABB01927, IHC(P)
IHC(P): Mouse Thymus Tissue

Anti-SQSTM1 Picoband antibody, OABB01927, IHC(P)
IHC(P): Rat Spleen Tissue

Anti-SQSTM1 Picoband antibody, OABB01927, IHC(F)
IHC(F): Rat Spleen Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Monkey, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for SQSTM1/p62 is approximately 0.1ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: DEDGDLVAFSSDEELTMAMSYVKDDIFR
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Intended Use: For research use only.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat, Monkey
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Mouse, Rat
  Immunocytochemistry: 0.5-1 ug/ml: Human
  Immunofluorescence: 2 ug/ml: Human
• Reference: 1. Hocking, L. J., Lucas, G. J. A., Daroszewska, A., Mangion, J., Olavesen, M., Cundy, T., Nicholson, G. C., Ward, L., Bennett, S. T., Wuyts, W., Van Hul, W., Ralston, S. H. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease. Hum. Molec. Genet. 11: 2735-2739, 2002.
  2. Joung, I., Strominger, J. L., Shin, J. Molecular cloning of a phosphotyrosine-independent ligand of the p56-lck SH2 domain. Proc. Nat. Acad. Sci. 93: 5991-5995, 1996.
  3. Park, I., Chung, J., Walsh, C. T., Yun, Y., Strominger, J. L., Shin, J. Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56-lck and its regulation by phosphorylation of ser-59 in the lck unique N-terminal region. Proc. Nat. Acad. Sci. 92: 12338-12342, 1995.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, IHC-F, ICC, IF in Human;Mouse;Monkey;Rat.

Target Info

• Gene Symbol: SQSTM1
• Gene Full Name: sequestosome 1
• Alias Symbols: A170;autophagy receptor p62;DMRV;EBI3-associated protein of 60 kDa;EBI3-associated protein p60;EBIAP;FTDALS3;NADGP;OSIL;oxidative stress induced like;p60;p62;p62B;PDB3;phosphotyrosine independent ligand for the Lck SH2 domain p62;phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;sequestosome-1;ubiquitin-binding protein p62;ZIP3.
• NCBI Gene Id: 8878
• Protein Name: Sequestosome-1
• Description of Target: Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102).
• Uniprot ID: Q13501
• Molecular Weight: 47687 MW