SQSTM1 Antibody (OAAB18212)

Catalog No: OAAB18212

Size:400ul

Western blot analysis of lysates from Hela cell line and mouse liver tissue lysate (from left to right), using Sqstm1 Antibody (S351) .
ctrl (Cat. #OAAB18212).
OAAB18212 was diluted at 1:1000 at each lane.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
  Cellular Location:Cytoplasm. Late endosome. Nucleus. Endoplasmic reticulum. Lysosome. Cytoplasmic vesicle, autophagosome. Cytoplasm, P-body. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. May also localize to the hepatocellular carcinoma. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates (By similarity) Co-localizes with TRIM5 in the cytoplasmic bodies
  Tissue Location: Widely expressed.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Phospho-Sqstm1(S351) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 346-379 amino acids from the region of human Phospho-Sqstm1(S351).
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Intended Use: Sqstm1(S351) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
• Reference: Ishii T., et al.Biochem. Biophys. Res. Commun. 226:456-460(1996).
  Morris J.C., et al.Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
  Carninci P., et al.Science 309:1559-1563(2005).
  Church D.M., et al.PLoS Biol. 7:E1000112-E1000112(2009).
  Ishii T., et al.Biochem. Biophys. Res. Commun. 232:33-37(1997).

Target Info

• Gene Symbol: SQSTM1
• Alias Symbols: S, p6, A17, OSF, Osi, p62, A170, STAP, OSF-6, STONE14
• NCBI Gene Id: 18412
• Description of Target: Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD.
• Uniprot ID: Q64337, O08623, Q13501
• Molecular Weight: 48163 Da