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Catalog No: OKCD00483 (Formerly GWB-KBBCL2)
Size:96WELLS
Price: $675.00
SKU
OKCD00483
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Datasheets/ManualsPrintable datasheet for SPTBN4 ELISA Kit (Human) (OKCD00483)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spectrin Beta, Non Erythrocytic 4 (SPTbN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spectrin Beta, Non Erythrocytic 4 (SPTbN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spectrin Beta, Non Erythrocytic 4 (SPTbN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Spectrin Beta, Non Erythrocytic 4 (SPTbN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spectrin Beta, Non Erythrocytic 4 (SPTbN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spectrin Beta, Non Erythrocytic 4 (SPTbN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SPTBN4 Microplate96 Wells (12 x 8 Well Strips)
SPTBN4 Lyophilized Standard2 x 10 ng
100X Biotinylated SPTBN4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Spectrin Beta, Non Erythrocytic 4 (SPTbN4). No significant cross-reactivity or interference between Spectrin Beta, Non Erythrocytic 4 (SPTbN4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSPTBN4
Gene Full Namespectrin beta, non-erythrocytic 4
Alias Symbolsbeta-IV spectrin;CMND;NEDHND;QV;spectrin beta chain, brain 3;spectrin beta chain, non-erythrocytic 4;spectrin, non-erythroid beta chain 3;SPNB4;SPTBN3.
NCBI Gene Id57731
Protein NameSpectrin beta chain, non-erythrocytic 4
Description of TargetSpectrin beta chain, non-erythrocytic 4
Uniprot IDQ9H254
Protein Accession #NP_066022
Nucleotide Accession #NM_020971.2
Protein Size (# AA)2564
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