SPTAN1 ELISA Kit (Mouse) (OKCD00657)

Catalog No: OKCD00657 (Formerly GWB-KBBGZ7)

Size:96WELLS

Mouse Sptan1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fodrin (SPTAN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fodrin (SPTAN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fodrin (SPTAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-Fodrin (SPTAN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fodrin (SPTAN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fodrin (SPTAN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Sptan1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Sptan1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated Sptan1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 0.68 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Fodrin (SPTAN1). No significant cross-reactivity or interference between Alpha-Fodrin (SPTAN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SPTAN1
• Gene Full Name: spectrin alpha, non-erythrocytic 1
• Alias Symbols: Alpha-II spectrin;Fodrin alpha chain.
• NCBI Gene Id: 20740
• Protein Name: Spectrin alpha chain, non-erythrocytic 1
• Description of Target: Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
• Uniprot ID: P16546
• Protein Accession #: NP_001171138
• Nucleotide Accession #: NM_001177667.1
• Protein Size (# AA): 2472