SPRY4 Antibody (OAAB04476)

Catalog No: OAAB04476

Size:400ul

Western blot analysis of Phospho-SPRY4-Y75.ctrl in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.

SPRY4-Y75 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
  Function: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
  Cellular Location: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human SPRY4.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  SPRY4 is supported by BioGPS gene expression data to be expressed in K562

• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: SPRY4
• Alias Symbols: HH17
• NCBI Gene Id: 81848
• Protein Name: Protein sprouty homolog 4
• Uniprot ID: Q9C004
• Protein Accession #: NP_001120968.1
• Molecular Weight: 32541 Da