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Datasheets/Manuals | Printable datasheet for Spred3 Antibody (OAPB00771) (OAPB00771) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Spred3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | Spred3 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3. The immunogen is located within amino acids 140 - 190 of Spred3. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-Spred3 antibody is Catalog # OEPB02895 |
Application Info | Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 ug/ml. (Optimal dilution should be determined by user) |
Reference | Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.;Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.;Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.;Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33. |
Gene Symbol | SPRED3 |
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Gene Full Name | sprouty related EVH1 domain containing 3 |
Alias Symbols | Eve-3;spred-3;sprouty-related, EVH1 domain-containing protein 3. |
NCBI Gene Id | 399473 |
Protein Name | Sprouty-related, EVH1 domain-containing protein 3|sprouty-related, EVH1 domain-containing protein 3 [Homo sapiens] |
Description of Target | Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. |
Uniprot ID | Q2MJR0 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001035987 |
Molecular Weight | Predicted: 45 kDa Observed: 45 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Spred3 Antibody (OAPB00771)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Spred3 Antibody (OAPB00771)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Spred3 Antibody (OAPB00771)" provided in?
This item is provided in "Liquid. Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Spred3 Antibody (OAPB00771)"?
This target may also be called "Eve-3;spred-3;sprouty-related, EVH1 domain-containing protein 3." in publications.
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What is the shipping cost for "Spred3 Antibody (OAPB00771)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Spred3 Antibody (OAPB00771)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Spred3 Antibody (OAPB00771)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 45 kDa Observed: 45 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Spred3 Antibody (OAPB00771)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SPRED3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SPRED3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SPRED3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SPRED3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SPRED3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SPRED3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.