Catalog No: OKCD01023 (Formerly GWB-KBBGZ3)
Size:96WELLS
Price: $675.00
SKU
OKCD01023
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Datasheets/ManualsPrintable datasheet for SPR ELISA Kit (Human) (OKCD01023)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase (SPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sepiapterin Reductase (SPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase (SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sepiapterin Reductase (SPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SPR Microplate96 Wells (12 x 8 Well Strips)
SPR Lyophilized Standard2 x 20 ng
100X Biotinylated SPR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sepiapterin Reductase (SPR). No significant cross-reactivity or interference between Sepiapterin Reductase (SPR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSPR
Gene Full Namesepiapterin reductase
Alias SymbolsSDR38C1;sepiapterin reductase;sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase);Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming);short chain dehydrogenase/reductase family 38C, member 1.
NCBI Gene Id6697
Protein NameSepiapterin reductase
Description of TargetCatalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
Uniprot IDP35270
Protein Accession #NP_003115
Nucleotide Accession #NM_003124.4
Protein Size (# AA)261
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