SPP2 ELISA Kit (Human) (OKCD01143)

Catalog No: OKCD01143 (Formerly GWB-KBBPE9)

Size:96WELLS

Human SPP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-103%	80-95%	85-101%	79-90%
  EDTA plasma(n=5)	82-89%	91-99%	96-104%	82-92%
  heparin plasma(n=5)	80-101%	89-96%	83-104%	83-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Phosphoprotein 2 (SPP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Phosphoprotein 2 (SPP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Phosphoprotein 2 (SPP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secreted Phosphoprotein 2 (SPP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-97	82
  EDTA plasma(n=5)	91-98	94
  heparin plasma(n=5)	91-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Phosphoprotein 2 (SPP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Phosphoprotein 2 (SPP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SPP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • SPP2 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated SPP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 26.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). No significant cross-reactivity or interference between Secreted Phosphoprotein 2 (SPP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SPP2
• Gene Full Name: secreted phosphoprotein 2
• Alias Symbols: Secreted phosphoprotein 2;secreted phosphoprotein 2, 24kD;secreted phosphoprotein 2, 24kDa;secreted phosphoprotein 24;SPP24;SPP-24.
• NCBI Gene Id: 6694
• Protein Name: Secreted phosphoprotein 24
• Description of Target: Could coordinate an aspect of bone turnover.
• Uniprot ID: Q13103
• Protein Accession #: NP_008875
• Nucleotide Accession #: NM_006944.2
• Protein Size (# AA): 211