Spp1 ELISA Kit (Rat) (OKIA00198)

Catalog No: OKIA00198

Size:1 x 96 well plate

Representative Rat Spp1 Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Rat
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 200 Minutes
• ELISA Kit Principle: The Aviva Systems Biology Spp1 ELISA Kit (Rat) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Osteopontin in Plasma or Serum. This assay employs an antibody specific for Rat Spp1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Spp1 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Spp1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of Spp1 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 0.07 - 4 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Rat Spp1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Rat Spp1 Calibrator: 1
  • 100X Spp1 Detection Antibody: 150 uL
  • 100X Streptavidin-HRP Conjugate: 150 uL
  • 1X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The Spp1 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Spp1/Osteopontin in Rat biological samples.
  Sample Volume: 2 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.03 ng/mL

Target Info

• Alias Symbols: Spp1;SPP-1;2b7;OPN;osteopontin;bone sialoprotein 1;secreted phosphoprotein 1
• Protein Name: Osteopontin
• Description of Target: Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction (by similarity). Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
• Uniprot ID: P08721