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Catalog No: OKIA00203
Size:1 x 96 well plate
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityDog
Product Format12 of 8-well strips
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration170 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology SPP1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Osteopontin in Urine, Plasma, or Serum. This assay employs an antibody specific for Canine SPP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-SPP1 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of SPP1 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range0.16 - 10 ng/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
Anti-Canine SPP1 Microplate96 Wells (12 x 8 Well Strips)
Canine SPP1 Calibrator1
100X SPP1 Detection Antibody150 uL
100X Streptavidin-HRP Conjugate150 uL
1X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The SPP1 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SPP1/Osteopontin in Canine biological samples.
::Sample Volume: 20 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity0.1 ng/mL
Alias SymbolsSpp1;OPN;osteopontin;bone sialoprotein 1;secreted phosphoprotein 1;
Protein NameOsteopontin
Description of TargetMajor non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.By Similarity Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
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