Catalog No: OKIA00203
Size:1 x 96 well plate
Price: $525.00
SKU
OKIA00203
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Dog | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Product Format | 12 of 8-well strips | ||||||||||||||||||
Application | ELISA | ||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||
ELISA Kit Duration | 170 Minutes | ||||||||||||||||||
ELISA Kit Principle | The Aviva Systems Biology SPP1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Osteopontin in Urine, Plasma, or Serum. This assay employs an antibody specific for Canine SPP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-SPP1 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of SPP1 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution. | ||||||||||||||||||
ELISA Kit Range | 0.16 - 10 ng/mL | ||||||||||||||||||
ELISA Kit Reproducibility | < 10% | ||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||
Additional Information | Intended Use: The SPP1 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SPP1/Osteopontin in Canine biological samples. | ||||||||||||||||||
:: | Sample Volume: 20 uL | ||||||||||||||||||
Reconstitution and Storage | Store at 4 degrees | ||||||||||||||||||
Sensitivity | 0.1 ng/mL |
Alias Symbols | Spp1;OPN;osteopontin;bone sialoprotein 1;secreted phosphoprotein 1; |
---|---|
Protein Name | Osteopontin |
Description of Target | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.By Similarity Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review