SPP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03843)

Catalog No: OKCD03843

Size:96WELLS

Human SPP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-102%	88-96%	93-101%	87-96%
  EDTA Plasma (n=5)	80-102%	99-105%	97-105%	82-99%
  Heparin Plasma (n=5)	95-104%	90-104%	95-102%	78-90%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteopontin (OPN) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-104	101
  EDTA Plasma (n=5)	91-104	95
  Heparin Plasma (n=5)	81-90	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SPP1 Microplate: 96 Wells (12 x 8 Well strips)
  • SPP1 Lyophilized Standard: 2
  • 100X SPP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.14 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SPP1
• Gene Full Name: secreted phosphoprotein 1
• Alias Symbols: BNSP;Bone sialoprotein 1;BSPI;early T-lymphocyte activation 1;ETA-1;nephropontin;OPN;osteopontin;osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein;Secreted phosphoprotein 1;secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1);secreted phosphoprotein 1 variant 6;SPP1/CALPHA1 fusion;urinary stone protein;uropontin.
• NCBI Gene Id: 6696
• Protein Name: Osteopontin
• Description of Target: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
• Uniprot ID: P10451
• Protein Accession #: NP_000573
• Nucleotide Accession #: NM_000582