SPOCK2 ELISA Kit (Human) (OKCD09363)

Catalog No: OKCD09363

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Testican 2 (TIC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Testican 2 (TIC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Testican 2 (TIC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Testican 2 (TIC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testican 2 (TIC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testican 2 (TIC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SPOCK2 Microplate: 96 Wells (12 x 8 Well strips)
  • SPOCK2 Lyophilized Standard: 2
  • 100X SPOCK2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.132ng/mL

Target Info

• Gene Symbol: SPOCK2
• Gene Full Name: SPARC (osteonectin), cwcv and kazal like domains proteoglycan 2
• Alias Symbols: SPARC/osteonectin, cwcv and kazal like domains proteoglycan 2;sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2;SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2;testican-2.
• NCBI Gene Id: 9806
• Protein Name: Testican-2
• Description of Target: May participate in diverse steps of neurogenesis. Binds calcium.
• Uniprot ID: Q92563
• Protein Accession #: NP_001127906.1
• Nucleotide Accession #: NM_001134434.1