SPN Antibody - middle region (OAAB06219)

Catalog No: OAAB06219

Size:400UL

Western blot analysis of SPN Antibody (Center) in HL-60 cell lysates (35ug/lane). SPN (arrow) was detected using the purified Pab.

SPN is strongly supported by BioGPS gene expression data to be expressed in HL60

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
  Description: This SPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Central region of human SPN.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the Central region of human SPN.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  SPN is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:8000

Target Info

• Gene Symbol: SPN
• Alias Symbols: CD43, galactoglycoprotein, GALGP, GPL115, leukocyte sialoglycoprotein, leukosialin, LSN, Sialophorin, sialophorin (gpL115, leukosialin, CD43).
• NCBI Gene Id: 6693
• Protein Name: Leukosialin|leukosialin precursor [Homo sapiens]
• Description of Target: Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
• Uniprot ID: P16150
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001025459.1,NP_003114.1
• Molecular Weight: 40322