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SPIRE1 Antibody - C-terminal region (OAAB12896)
Datasheets/Manuals | Printable datasheet for SPIRE1 Antibody (C-term) (OAAB12896) |
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Predicted Species Reactivity | Human, Mouse, Primate |
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Product Format | Liquid.PBS with 0.09% (W/V) sodium azide |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | Western blot |
Additional Information | Cellular Location: Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion. Punctate spots in perinuclear region and cytoplasm, colocalized with Rab11. |
Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This SPIRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 665-691 amino acids from the C-terminal region of human SPIRE1. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Concentration | 0.34 mg/ml |
Application Info | WB: 1:1000 Optimal dilutions should be determined by the end user. |
Reference | Rose, J. Phd, et al. Mol. Med. (2010) In press Pechlivanis, M., et al. J. Biol. Chem. 284(37):25324-25333(2009) Bosch, M., et al. Mol. Cell 28(4):555-568(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) Kerkhoff, E., et al. Curr. Biol. 11(24):1963-1968(2001) |
Publications | Correction: DNA damage induces nuclear actin filament assembly by Formin-2 and Spire-1/2 that promotes efficient DNA repair. Elife. 4, (2015). 26437453 DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected]. Elife. 4, e07735 (2015). 26287480 |
Description |
Gene Symbol | SPIRE1 |
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Gene Full Name | spire type actin nucleation factor 1 |
Alias Symbols | protein spire homolog 1;Spir-1;spire actin nucleation factor 1;spire family actin nucleation factor 1;spire homolog 1. |
NCBI Gene Id | 56907 |
Protein Name | NP_001122098.1, NP_001122099.1, NP_064533.3, Q08AE8, Q4R707, Q52KF3 |
Description of Target | Protein spire homolog 1, protein spire homolog 1 isoform a [Homo sapiens], protein spire homolog 1 isoform b [Homo sapiens], protein spire homolog 1 isoform c [Homo sapiens] |
Uniprot ID | https://www.uniprot.org/uniprot/?query=Q08AE8+OR+Q4R707+OR+Q52KF3 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001122098.1,NP_001122099.1,NP_064533.3 |
Molecular Weight | 85544 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Primate".
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How long will it take to receive "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks ".
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What buffer format is "SPIRE1 Antibody - C-terminal region (OAAB12896)" provided in?
This item is provided in "Liquid.PBS with 0.09% (W/V) sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
This target may also be called "protein spire homolog 1;Spir-1;spire actin nucleation factor 1;spire family actin nucleation factor 1;spire homolog 1." in publications.
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What is the shipping cost for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "85544".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SPIRE1 Antibody - C-terminal region (OAAB12896)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SPIRE1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SPIRE1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SPIRE1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SPIRE1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SPIRE1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SPIRE1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.