Catalog No: OKEH03929
Size:96T
Price: $625.00
SKU
OKEH03929
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SPINK1 ELISA Kit (Pig) (OKEH03929) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPINK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPINK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPINK1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Component
ComponentAmount
SPINK1 Microplate96 Wells (12 x 8 Well strips)
SPINK1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SPINK1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.28 ng/mL
SpecificityNatural and recombinant Pig Serine protease inhibitor Kazal-type 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSPINK1
Gene Full Nameserine peptidase inhibitor, Kazal type 1
Alias SymbolsPancreatic secretory trypsin inhibitor.
NCBI Gene Id100719912
Protein NameP00998
Description of TargetSerine protease inhibitor Kazal-type 1
Uniprot IDhttps://www.uniprot.org/uniprot/P00998
Protein Accession #XP_003477374.1
Nucleotide Accession #XM_003477326.2
Protein Size (# AA)56
Molecular Weight56 kDa
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