Catalog No: OKBB00557
Size:96 Wells
Price: $876.00
SKU
OKBB00557
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human SPINK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPINK1 has been precoated onto 96-well plates. Standards(E.coli, D24-C79) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPINK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human SPINK1 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.32.74.81.62.45.1
SD0.0520.1380.2740.120.130.332
CV%45.15.77.55.46.5
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human SPINK1 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human SPINK1 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human SPINK1 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin).
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human SPINK1
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: D24-C79
DescriptionFor quantitative detection of human SPINK1 in cell culture supernatants, serum and plasma(heparin).
Gene SymbolSPINK1
Gene Full Nameserine peptidase inhibitor, Kazal type 1
Alias SymbolsISK1_HUMAN, Pancreatic secretory trypsin inhibitor, Pancreatic serine protease inhibitor, Kazal, PCTT, PSTI, Serine protease inhibitor Kazal type 1, Serine protease inhibitor Kazal-type 1, Spink 3, SPINK1, Spink3, TaTI, TCP, Tumor associated trypsin inhibitor, Tumor-associated trypsin inhibitor
NCBI Gene Id6690
Protein NamePancreatic secretory trypsin inhibitor
Description of TargetSerine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
Uniprot IDP00995
Protein Accession #NP_003113.2
Nucleotide Accession #NM_003122.4
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