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| Datasheets/Manuals | Printable datasheet for SPHK1 Antibody (OAEB02487) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Rat |
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB, P-ELISA, IP |
| Reconstitution and Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Immunogen | SPHK1 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Peptide Sequence | C-DVDLESEKYRRLGE |
| Concentration | 0.5 mg/ml |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1). |
| Application Info | P-ELISA: Antibody detection limit dilution 1:16000 WB: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 ug/ml. IP: OAEB00969 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected. |
| Reference | * Igarashi N, Okada T, Hayashi S, Fujita T, Jahangeer S, Nakamura S.; Sphingosine kinase 2 is a nuclear protein and inhibits DNA synthesis.; J Biol Chem. 2003 Nov 21;278(47):46832-9. Epub 2003 Sep 2.; Pubmed 12954646 * Kase H, Hattori Y, Jojima T, Okayasu T, Tomizawa A, Suzuki K, Banba N, Monden T, Satoh H, Akimoto K, Kasai K.; Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells.; Life Sci. 2007 Aug 23;81(11):939-43. Epub 2007 Aug 17.; Pubmed 17822721 |
| Gene Symbol | Sphk1|SPHK1 |
|---|---|
| Gene Full Name | sphingosine kinase 1 |
| Alias Symbols | 1110006G24Rik;acetyltransferase SPHK1;SK;SK 1;Sk1;sphingosine kinase 1;SPHK;SPK 1;Spk1. |
| NCBI Gene Id | 170897|20698|8877 |
| Protein Name | Sphingosine kinase 1 EMBL AAH09419.1 |
| Uniprot ID | Q96GK1 |
| Protein Accession # | NP_068807.2 |
| Molecular Weight | 43.9; 51.1; 42.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SPHK1 Antibody (OAEB02487)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Rat".
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How long will it take to receive "SPHK1 Antibody (OAEB02487)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "SPHK1 Antibody (OAEB02487)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SPHK1 Antibody (OAEB02487)"?
This target may also be called "1110006G24Rik;acetyltransferase SPHK1;SK;SK 1;Sk1;sphingosine kinase 1;SPHK;SPK 1;Spk1." in publications.
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What is the shipping cost for "SPHK1 Antibody (OAEB02487)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SPHK1 Antibody (OAEB02487)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SPHK1 Antibody (OAEB02487)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "43.9; 51.1; 42.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SPHK1 Antibody (OAEB02487)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Sphk1|SPHK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Sphk1|SPHK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Sphk1|SPHK1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Sphk1|SPHK1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Sphk1|SPHK1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Sphk1|SPHK1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
