SPHK1 Antibody - middle region (OAAB18595)

Catalog No: OAAB18595

Size:200ul

The anti-SPHK1 Pab (Cat. #OAAB18595) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
Dilution: 1:1000

Western blot analysis of anti-SPHK1 Antibody (Center) (Cat. #OAAB18595) in 293 cell line lysates (35ug/lane).
SPHK1 (arrow) was detected using the purified Pab.
Dilution: 1:1000

Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) (Cat. #OAAB18595).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Dilution: 1:1000

Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301) (Cat. #OAAB18595).
OAAB18595 was diluted at 1:1000 at each lane.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.
Dilution: 1:1000

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BC = breast carcinoma; HC = hepatocarcinoma.
Dilution: 1:50~100

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Application Info: IHC-P: 1:25
  WB: 1:2000
  WB: 1:1000
• Reference: Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000). Melendez, A.J., et al., Gene 251(1):19-26 (2000). Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).

Target Info

• Gene Symbol: SPHK1
• Alias Symbols: SPHK
• NCBI Gene Id: 8877
• Description of Target: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
• Uniprot ID: Q9NYA1
• Protein Accession #: NP_001136073.1, NP_001136074.1, NP_068807.2, NP_892010.2
• Molecular Weight: 43 kDa