SPARC ELISA Kit (Human) (OKBB00542)

Catalog No: OKBB00542

Size:96 Wells

Human SPARC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human SPARC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARC has been precoated onto 96-well plates. Standards(NS0, A18-I303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human SPARC amount of sample captured in plate.
• ELISA Kit Range: 0.78 ng/ml - 50 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	7	19.5	32.2	10.1	24.5	38.4
  SD	0.357	0.819	1.26	0.596	1.568	2.04
  CV%	5.1	4.2	4.8	5.9	7.4	5.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human SPARC Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human SPARC Standard: 2 x 50 ng/tube
  • 100X Biotinylated Anti-Human SPARC Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and human milk
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant human SPARC
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A18-I303
• Description: For quantitative detection of human SPARC in cell culture supernatants, serum and human milk.

Target Info

• Gene Symbol: SPARC
• Gene Full Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Alias Symbols: AA517111, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, BM40, Cysteine rich protein, MGC128090, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and rich in cysteine, Secreted protein acidic cysteine rich (osteonectin), Secreted protein acidic cysteine rich, SPARC, SPRC, SPRC_HUMAN
• NCBI Gene Id: 6678
• Protein Name: SPARC
• Description of Target: SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
• Uniprot ID: P09486
• Protein Accession #: NP_001296372.1
• Nucleotide Accession #: NM_001309443.1