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Datasheets/Manuals | Printable datasheet for anti-SPARC antibody - OAAB03818 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Host | Rabbit |
Application | WB, IHC-P, FC |
:: | Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Cellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus |
Concentration | 2 mg/ml |
Application Info | WB: 1:1000 IHC-P: 1:10~50 FC: 1:10~50 |
Other Applications Image 1 Data | Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Gene Symbol | SPARC |
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Alias Symbols | ON, ONT, OI17, BM-40 |
NCBI Gene Id | 6678 |
Protein Name | SPARC |
Description of Target | Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM). |
Uniprot ID | P09486 |
Protein Accession # | NP_003109.1 |
Molecular Weight | 35 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SPARC Antibody - C-terminal region (OAAB03818)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "SPARC Antibody - C-terminal region (OAAB03818)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "SPARC Antibody - C-terminal region (OAAB03818)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SPARC Antibody - C-terminal region (OAAB03818)"?
This target may also be called "ON, ONT, OI17, BM-40" in publications.
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What is the shipping cost for "SPARC Antibody - C-terminal region (OAAB03818)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SPARC Antibody - C-terminal region (OAAB03818)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SPARC Antibody - C-terminal region (OAAB03818)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "35 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SPARC Antibody - C-terminal region (OAAB03818)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SPARC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SPARC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SPARC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SPARC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SPARC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SPARC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.