SPAG9 Antibody - middle region (OAAB04263)

Catalog No: OAAB04263

Size:400ul

Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected with the SPAG9 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P, WB
• Additional Information: Cellular Location:  Cytoplasm. Cytoplasm, perinuclear region. Note=Perinuclear distribution in response to stress signals such as UV radiation
  Tissue Location:  Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the Central region of human JLP (SPAG9).
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: SPAG9
• Alias Symbols: JLP, CT89, HLC4, HLC6, JIP4, PHET, PIG6, HLC-6, JIP-4
• NCBI Gene Id: 9043
• Protein Name: C-Jun-amino-terminal kinase-interacting protein 4
• Description of Target: SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
• Uniprot ID: O60271
• Protein Accession #: NP_001123999.1
• Molecular Weight: 146205 Da