SPAG8 Antibody - N-terminal region (ARP55621_P050)

Catalog No: ARP55621_P050

WB Suggested Anti-SPAG8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Liver

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rabbit, Yeast
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SPAG8
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Rabbit: 86%; Yeast: 93%
• Peptide Sequence: Synthetic peptide located within the following region: SGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSH
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-SPAG8 (ARP55621_P050) antibody is Catalog # AAP55621 (Previous Catalog # AAPP35544)
• Reference: Cheng,G.Y., (2007) Asian J. Androl. 9 (1), 23-29

Target Info

• Gene Symbol: SPAG8
• Gene Full Name: Sperm associated antigen 8
• Alias Symbols: SMP1, BS-84, CT142, HSD-1, SPAG3, CILD28, hSMP-1
• NCBI Gene Id: 26206
• Protein Name: Sperm-associated antigen 8
• Description of Target: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
• Uniprot ID: Q99932
• Protein Accession #: NP_758516
• Nucleotide Accession #: NM_172312
• Protein Size (# AA): 501
• Molecular Weight: 53kDa
• Protein Interactions: KLHL32; DTX2; SNRPC; CSTF2; PITX1; JMJD4; WHSC1L1; KDM3B; CARM1; PRMT5; PRMT1; PRMT2; RANBP9; UBQLN4; NEDD4L; RAN;