SP140 ELISA Kit (Human) (OKCD00627)

Catalog No: OKCD00627 (Formerly GWB-KBBNV7)

Size:96WELLS

Human SP140 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sp140 Nuclear Body Protein (SP140). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sp140 Nuclear Body Protein (SP140). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sp140 Nuclear Body Protein (SP140), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sp140 Nuclear Body Protein (SP140) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sp140 Nuclear Body Protein (SP140) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sp140 Nuclear Body Protein (SP140) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SP140 Microplate: 96 Wells (12 x 8 Well Strips)
  • SP140 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SP140 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sp140 Nuclear Body Protein (SP140). No significant cross-reactivity or interference between Sp140 Nuclear Body Protein (SP140) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SP140
• Gene Full Name: SP140 nuclear body protein
• Alias Symbols: lymphoid-restricted homolog of Sp100;lymphoid-specific SP100 homolog;LYSP100;LYSP100-A;LYSP100-B;nuclear autoantigen Sp-140;nuclear body protein SP140;speckled 140 kDa.
• NCBI Gene Id: 11262
• Protein Name: Nuclear body protein SP140
• Description of Target: Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body (PubMed:8910577). May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection (PubMed:8910577). May play a role in chromatin-mediated regulation of gene expression although it does not bind to histone H3 tails (PubMed:24267382).
• Uniprot ID: Q13342
• Protein Accession #: NP_001005176
• Nucleotide Accession #: NM_001005176.2
• Protein Size (# AA): 867