SOX9 ELISA Kit (Human) (OKCD08915)

Catalog No: OKCD08915

Size:96WELLS

SOX9 ELISA Kit (Human) (OKCD08915) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Determining Region Y Box Protein 9 (SOX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sex Determining Region Y Box Protein 9 (SOX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SOX9 Microplate: 96 Wells (12 x 8 Well strips)
  • SOX9 Lyophilized Standard: 2
  • 100X SOX9 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.115ng/mL

Target Info

• Gene Symbol: SOX9
• Gene Full Name: SRY-box transcription factor 9
• Alias Symbols: CMD1;CMPD1;SRA1;SRXX2;SRXY10;SRY (sex determining region Y)-box9;SRY (sex-determining region Y)-box 9 protein;SRY-box 9;SRY-related HMG-box, gene 9;transcription factor SOX-9.
• NCBI Gene Id: 6662
• Protein Name: Transcription factor SOX-9
• Description of Target: Transcriptional regulator (PubMed:24038782). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (By similarity). Plays a role in chondrocyte differentiation (By similarity). Important for normal skeletal development (PubMed:24038782).
• Uniprot ID: P48436
• Protein Accession #: NP_000337.1
• Nucleotide Accession #: NM_000346.3