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SOX17 Antibody (OAEB00408)

100 ug
$229.00
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Gene Symbol:
SOX17
NCBI Gene Id:
64321
Protein Name:
Transcription factor SOX-17
Swissprot Id:
Q9H6I2
Protein Accession #:
NP_071899.1
Replacement Item:
This antibody may replace item sc-130295 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SOX17.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SOX17.
Predicted Species Reactivity:
Dog, Guinea Pig, Human, Mouse, Rat
Predicted Homology Based on Immunogen Sequence:
Dog: 90%; Guinea Pig: 100%; Human: 100%; Mouse: 90%; Rat: 90%
Complete computational species homology data:
SOX17 Antibody (OAEB00408)
Peptide Sequence:
EALPCRDGTDP
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-SOX17 antibody - OAEB00408
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Preliminary experiments gave an approx 20kDa band in Rat Brain and Rat Spinal Cord lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.6kDa according to Mouse NP_035571.1 and of 44.1kDa according to Human NP_071899.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide.
Target Reference:
* Kim I, Saunders TL, Morrison SJ.; Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells.; Cell. 2007 Aug 10;130(3):470-83. Epub 2007 Jul 26.; Pubmed 17655922

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