SOSTDC1 ELISA Kit (Human) (OKCD08859)

Catalog No: OKCD08859

Size:96WELLS

SOSTDC1 ELISA Kit (Human) (OKCD08859) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sclerostin Domain Containing Protein 1 (SOSTDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sclerostin Domain Containing Protein 1 (SOSTDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sclerostin Domain Containing Protein 1 (SOSTDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sclerostin Domain Containing Protein 1 (SOSTDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sclerostin Domain Containing Protein 1 (SOSTDC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sclerostin Domain Containing Protein 1 (SOSTDC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SOSTDC1 Microplate: 96 Wells (12 x 8 Well strips)
  • SOSTDC1 Lyophilized Standard: 2
  • 100X SOSTDC1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: SOSTDC1
• Gene Full Name: sclerostin domain containing 1
• Alias Symbols: CDA019;cystine-knot containing secreted protein;DAND7;ectodermal BMP inhibitor;ECTODIN;sclerostin domain-containing protein 1;USAG1;Uterine sensitization-associated gene 1 protein;uterine sensitization-associated protein-1;wise.
• NCBI Gene Id: 25928
• Protein Name: Sclerostin domain-containing protein 1
• Description of Target: May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction Enhances Wnt signaling and inhibits TGF-beta signaling (By similarity). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.
• Uniprot ID: Q6X4U4
• Protein Accession #: NP_056279.1
• Nucleotide Accession #: NM_015464.2