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Catalog No: OKCD02875
Size:96WELLS
Price: $750.00
SKU
OKCD02875
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Datasheets/ManualsPrintable datasheet for SORD ELISA Kit (Bovine) (OKCD02875)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-94%86-104%80-101%94-101%
EDTA Plasma (n=5)95-103%82-97%89-96%79-89%
Heparin Plasma (n=5)86-94%78-92%94-103%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)96-105102
EDTA Plasma (n=5)92-9995
Heparin Plasma(n=5)90-9894
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SORD Microplate96 Wells (12 x 8 Well strips)
SORD Lyophilized Standard2
100X Biotinylated SORD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.12 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH). No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSORD
Gene Full Namesorbitol dehydrogenase
Alias SymbolsL-iditol 2-dehydrogenase;polyol dehydrogenase;SDH;sorbitol dehydrogenase;XDH;xylitol dehydrogenase.
NCBI Gene Id508954
Protein NameSorbitol dehydrogenase
Description of TargetConverts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
Uniprot IDQ58D31
Protein Accession #NP_001032397.1
Nucleotide Accession #NM_001037320.1
Protein Size (# AA)356
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