Catalog No: OKCD01289
Size:96WELLS
Price: $913.00
SKU
OKCD01289
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Somatostatin Antibody ELISA Kit (Pig) (OKCD01289)

Datasheets/ManualsPrintable datasheet for Somatostatin Antibody ELISA Kit (Pig) (OKCD01289)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h, 30min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-99%85-101%80-105%98-105%
EDTA Plasma (n=5)87-104%93-101%84-91%82-98%
Heparin Plasma (n=5)79-104%91-105%79-103%94-102%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Somatostatin Antibody (Anti-SST) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Somatostatin Antibody (Anti-SST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-9591
EDTA Plasma (n=5)87-9491
Heparin Plasma (n=5)92-10297
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Somatostatin antibody Microplate96 Wells (12 x 8 Well strips)
Somatostatin antibody Lyophilized Standard2 x XXX
100X Detector Antibody1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.27 ng/mL
Assay InfoAssay Method: Quantitative Reverse Capture Sandwich ELISA
Gene SymbolSST
Gene Full Namesomatostatin
Alias Symbolssomatostatin;SRIF.
NCBI Gene Id494469
Protein NameSomatostatin
Description of TargetSomatostatin inhibits the release of somatotropin.
Uniprot IDP01168
Nucleotide Accession #NM_001009583.1
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