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Catalog No: OKBB00546
Size:96 Wells
Price: $583.00
SKU
OKBB00546
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD6 has been precoated onto 96-well plates. Standards(NS0, H18-E398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD6 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.223.176.371.243.246.49
SD0.0650.1490.1780.0860.2040.376
CV%5.34.72.86.96.35.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human soluble CD6 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human soluble CD6 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human soluble CD6 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human CD6
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: H18-E398
DescriptionFor quantitative detection of human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolCD6
Gene Full NameCD6 molecule
Alias SymbolsCD_antigen=CD6, CD6, CD6 antigen Tp120, CD6 molecule, CD6_HUMAN, FLJ44171, OX52, T cell differentiation antigen CD6, T-cell differentiation antigen CD6, T12, TP120
NCBI Gene Id923
Protein NameT-cell differentiation antigen CD6
Description of TargetCD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
Uniprot IDP30203
Protein Accession #NP_001241679.1
Nucleotide Accession #NM_001254750.1
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