Sodium-Iodide Symporter Antibody (OASE00238)

Catalog No: OASE00238 (Formerly GWB-I98TR7)

Size:100ug

Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: 14F
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, AM
• Additional Information: Background Info: Apparent mol. wt of 97kD, non-glycosylated version at
  68kD. Other minor bands
  associated with hNIS at160
  kDa, and degradation products
  at ~30 kDa, and ~15kDa.
  Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral
  membrane into thyroid epithelial cells (1, 2). This is
  important step in the process of iodide organificaton and
  the formation of triiodothyronine and thyroxine (3).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Mannose binding protein hNIS fusion (AA468-643)
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa.
• Dilution: WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460.
  2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558.
  3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51):
  20250-20255.
• Description: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Target Info

• Gene Symbol: Sodium Iodide Symporter
• Gene Full Name: solute carrier family 5 (sodium/iodide cotransporter), member 5
• Alias Symbols: NIS, SLC5A5, solute carrier family 5, Na (+)I(-) cotransporter
• NCBI Gene Id: 6528
• Uniprot ID: Q92911
• Protein Accession #: NP_000444.1