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Datasheets/Manuals | Printable datasheet for OASE00238 |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Clonality | Monoclonal |
Clone | 14F |
Isotype | IgG1 |
Host | Mouse |
Conjugation | Unconjugated |
Application | WB, IHC, AM |
Additional Information | Background Info: Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160 kDa, and degradation products at ~30 kDa, and ~15kDa. |
:: | Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
Immunogen | Mannose binding protein hNIS fusion (AA468-643) |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Specificity | Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa. |
Dilution | WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user. |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Reference | 1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255. |
Description | The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). |
Gene Symbol | Sodium Iodide Symporter |
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Gene Full Name | solute carrier family 5 (sodium/iodide cotransporter), member 5 |
Alias Symbols | NIS, SLC5A5, solute carrier family 5, Na (+)I(-) cotransporter |
NCBI Gene Id | 6528 |
Uniprot ID | Q92911 |
Protein Accession # | NP_000444.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Sodium-Iodide Symporter Antibody (OASE00238)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Sodium-Iodide Symporter Antibody (OASE00238)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Sodium-Iodide Symporter Antibody (OASE00238)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Sodium-Iodide Symporter Antibody (OASE00238)"?
This target may also be called "NIS, SLC5A5, solute carrier family 5, Na (+)I(-) cotransporter" in publications.
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What is the shipping cost for "Sodium-Iodide Symporter Antibody (OASE00238)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Sodium-Iodide Symporter Antibody (OASE00238)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Sodium-Iodide Symporter Antibody (OASE00238)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Sodium-Iodide Symporter Antibody (OASE00238)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Sodium Iodide Symporter"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Sodium Iodide Symporter"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Sodium Iodide Symporter"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Sodium Iodide Symporter"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Sodium Iodide Symporter"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Sodium Iodide Symporter"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.