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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00239-Biotin |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Monoclonal |
Clone | FP5 |
Isotype | IgG1 Kappa |
Host | Mouse |
Conjugation | Biotin |
Application | WB, IHC, IF |
Additional Information | Background Info: Mapped to AA 625-643 of hNIS. Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160 kDa, and degradation products at ~30 kDa, and ~15kDa. |
:: | Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Mannose binding protein hNIS fusion (AA468-643) |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Dilution | 1:1000 (WB) |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255. |
Gene Symbol | SLC5A5 |
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Gene Full Name | solute carrier family 5 (sodium/iodide cotransporter), member 5 |
Alias Symbols | NIS, TDH1 |
NCBI Gene Id | 6528 |
Uniprot ID | Q92911 |
Protein Accession # | NP_000444.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
This target may also be called "NIS, TDH1" in publications.
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What is the shipping cost for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Sodium-Iodide Symporter Antibody : Biotin (OASE00239-Biotin)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SLC5A5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SLC5A5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SLC5A5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SLC5A5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SLC5A5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SLC5A5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.