Catalog No: OKEH01567
Size:96T
Price: $525.00
SKU
OKEH01567
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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SOD2 ELISA Kit (Human) : 96 Wells (OKEH01567)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH01567
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SOD2 ELISA Kit (Human) (OKEH01567) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SOD2 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
SOD2 Microplate96 Wells (12 x 8 Well strips)
SOD2 Lyophilized Standard2
100X Biotinylated SOD2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity12 pg/mL
SpecificityNatural and recombinant Human Superoxide dismutase [Mn], mitochondrial
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolSOD2
Gene Full Namesuperoxide dismutase 2
Alias Symbolsepididymis secretory sperm binding protein;gastric cancer-associated lncRNA 1;GClnc1;indophenoloxidase B;IPOB;IPO-B;manganese-containing superoxide dismutase;mangano-superoxide dismutase;Mn superoxide dismutase;Mn-SOD;MNSOD;MVCD6;superoxide dismutase [Mn], mitochondrial;superoxide dismutase 2, mitochondrial.
NCBI Gene Id6648
Protein NameP04179
Description of TargetSuperoxide dismutase [Mn], mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P04179
Protein Accession #NP_000627.2
Nucleotide Accession #NM_000636.2
Protein Size (# AA)222
Molecular Weight25 kDa
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