SOD2 Antibody (OAAB22144)

Catalog No: OAAB22144

Size:200ul

All lanes: SODM Antibody (OAAB22144) at 1:2000 dilution Lane 1:human brain lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OAAB22144 staining SODM in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

OAAB22144 staining SODM in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing A549 cells stained with OAAB22144 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (OAAB22144, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 1559CT572.3.83
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB
• Additional Information: Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SODM antibody is generated from a mouse immunized with a recombinant protein of human SODM.
• Purification: Protein G column purification, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 500 ug/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified monoclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: FC: 1:25
  IHC-P: 1:25
  WB: 1:2000
• Reference: Wispe J.R.,et al.Biochim. Biophys. Acta 994:30-36(1989).
  Beck Y.,et al.Nucleic Acids Res. 15:9076-9076(1987).
  Heckl K.,et al.Nucleic Acids Res. 16:6224-6224(1988).
  Ho Y.-S.,et al.FEBS Lett. 229:256-260(1988).
  Church S.L.,et al.Biochim. Biophys. Acta 1087:250-252(1990).
  

Target Info

• Gene Symbol: SOD2
• Gene Full Name: superoxide dismutase 2, mitochondrial
• Alias Symbols: IPOB, IPO-B, MNSOD, MVCD6, GClnc1, Mn-SOD
• NCBI Gene Id: 6648
• Description of Target: This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Uniprot ID: P04179
• Protein Accession #: NP_000627.2
• Nucleotide Accession #: NM_000636.2
• Molecular Weight: 25 kDa