SOD (EC) Antibody : HRP (OASE00103-HRP)

Catalog No: OASE00103-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: 4GG11G6
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, ELISA, IHC
• Additional Information: Background Info: Detects EC SOD at ~35kDa.
  Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4).
  There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however
  it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of EC-SOD in 20ug of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Human EC SOD purified from aortas
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:1000 (WB), 1:100 (IHC and ICC)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102.
  2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180.
  3. FurukawaY., and O'Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6.
  4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
  5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385.
  6. Wispe J.R., et al. (1989) BBA. 994: 30-36.
  7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
  

Target Info

• Gene Symbol: SOD3
• Gene Full Name: superoxide dismutase 3, extracellular
• Alias Symbols: EC-SOD
• NCBI Gene Id: 6649
• Uniprot ID: P08294
• Protein Accession #: NP_003093.2