SOCS1 Antibody - N-terminal region (OAAB05388)

Catalog No: OAAB05388

Size:400ul

Western blot analysis of SOCS1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC, IF, WB
  Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
  Cellular Location: Nucleus. Cytoplasmic vesicle. Note=Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.
  Tissue Location: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-66 amino acids from the N-terminal region of human SOCS1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: SOCS1
• Alias Symbols: JAB, CIS1, SSI1, TIP3, CISH1, SSI-1, TIP-3, SOCS-1
• NCBI Gene Id: 8651
• Protein Name: Suppressor of cytokine signaling 1
• Description of Target: SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling.
• Uniprot ID: O15524
• Protein Accession #: NP_003736.1
• Molecular Weight: 24 kDa