SNRNP70 ELISA Kit (Mouse) (OKEH07908)

Catalog No: OKEH07908

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SNRNP70 ELISA Kit (Mouse) (OKEH07908) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNRNP70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNRNP70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNRNP70 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.8% (n = 20)
  Mean Inter-assay CV%: <=9.3% (n = 20)
• ELISA Kit Component:
  • SNRNP70 Microplate: 96 Wells (12 x 8 Well strips)
  • SNRNP70 Lyophilized Standard: 2
  • 100X Biotinylated SNRNP70 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
  Subcellular Location: Nucleus speckle Nucleus Nucleoplasm Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.
  Unigene: Mm.216386
  SMR: Q62376
  String: 10090.ENSMUSP00000074160
  KEGG: mmu:20637
  Pfam: PF00076
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.172ng/mL
• Specificity: Natural and recombinant Mouse U1 small nuclear ribonucleoprotein 70 kDa
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Snrnp70
• Gene Full Name: small nuclear ribonucleoprotein 70 (U1)
• Alias Symbols: 2700022N21Rik;3200002N22Rik;AI325098;R74807;Rnu;Rnu1p70;Rnulp70;Sn;Snrp70;Sr;Srnp70;U1 small nuclear ribonucleoprotein 70 kDa;U1 small nuclear ribonucleoprotein 70 kDa polypeptide A;U1 snRNP 70 kDa;U1-70;U1-70K.
• NCBI Gene Id: 20637
• Protein Name: Q62376
• Description of Target: U1 small nuclear ribonucleoprotein 70 kDa
• Uniprot ID: https://www.uniprot.org/uniprot/Q62376
• Protein Accession #: NP_033250.3
• Nucleotide Accession #: NM_009224.5